Bringing It All Together: Coupling Excision Repair to the DNA Damage Checkpoint

Bringing It All Together: Bedside to Bench and Back Again.

DNA Repair: Keeping It Together

A protein scaffold has been identified that holds a chromosome together in the event of a DNA double-strand break. This scaffold is dependent on Rad52 and the Rad50-Mre11-Xrs2 complex and withstands the pulling forces of the mitotic spindle during DNA damage checkpoint arrest.

Bringing them all Together

A sequent calculus for hybrid logics is developed from a calculus for classical predicat logic by a series of transformations. We formalize the semantic theory of hybrid logic using a sequent calculus for predicate logic plus axioms. This works, but it is ugly. The unnattractive features are removed one-by-one, until the final vestiges of the metalanguage can be set aside to reveal a fully inte...

Base excision repair AP endonucleases and mismatch repair act together to induce checkpoint-mediated autophagy

Cellular responses to DNA damage involve distinct DNA repair pathways, such as mismatch repair (MMR) and base excision repair (BER). Using Caenorhabditis elegans as a model system, we present genetic and molecular evidence of a mechanistic link between processing of DNA damage and activation of autophagy. Here we show that the BER AP endonucleases APN-1 and EXO-3 function in the same pathway as...

Physical and functional interactions between nucleotide excision repair and DNA damage checkpoint.

The mechanisms used by checkpoints to identify DNA lesions are poorly understood and may involve the function of repair proteins. Looking for mutants specifically defective in activating the checkpoint following UV lesions, but proficient in the response to methyl methane sulfonate and double-strand breaks, we isolated cdu1-1, which is allelic to RAD14, the homolog of human XPA, involved in les...